Freeze-Fracture Enzyme Cytochemistry Reveals the Distribution of Enzymes in Biological Membranes: Enzyme Cytochemical Label-Fracture and Fracture-Label.
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چکیده
منابع مشابه
Freeze-fracture cytochemistry in cell biology.
The term freeze-fracture cytochemistry embraces a series of techniques which share the goal of chemical identification of the structural components viewed in freeze-fracture replicas. As one of the major features of freeze fracture is its ability to provide planar views of membranes, a major emphasis in freeze-fracture cytochemistry is to identify integral membrane proteins, study their spatial...
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Thin-section and critical-point-dried fracture-labeled preparations are used to determine the distribution and partition of glycophorin-associated wheat germ agglutinin (WGA) binding sites over protoplasmic and exoplasmic faces of freeze-fractured human erythrocyte membranes. Most wheat germ agglutinin binding sites are found over exoplasmic faces. Label is sparse over the protoplasmic faces. T...
متن کاملThe cell surface of Trypanosoma cruzi: cytochemistry and freeze-fracture.
The ultrastructure of epimastigotes of Trypanosoma cruzi, obtained from acellular cultures, and bloodstream trypomastigotes, isolated from infected mice, were studied by thin-sectioning and freeze-fracturing techniques. Epimastigotes showed a thin (5 nm) surface coat when stained with ruthenium red, while the surface coat of trypomastigotes was more prominent (15 nm thick). Both P and E faces o...
متن کاملFreeze-fracture cytochemistry: a new fracture-labeling method for topological analysis of biomembrane molecules.
Freeze-fracture cytochemistry allows visualization of cellular and molecular characteristics of biomembranes in situ. In this review, we discuss freeze-fracture cytochemistry with special reference to a new cytochemical labeling of replicas, the detergent-digestion fracture-labeling technique. In this procedure, unfixed cells are rapidly-frozen, freeze-fractured, and physically stabilized by ev...
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"Capaci ta t ion" is a physiological event which alters sperm to permit rapid penetration through oocyte investments and fusion between gametes. Acrosomal "reaction," the physiological release of acrosomal contents, occurs after this facilitating process. In this study, acrosomal "disruption" of guinea pig and rat sperm was achieved in vitro by incubating sperm together with the follicular cont...
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ژورنال
عنوان ژورنال: ACTA HISTOCHEMICA ET CYTOCHEMICA
سال: 1997
ISSN: 0044-5991,1347-5800
DOI: 10.1267/ahc.30.77